Health & Safety Industry Today

One Step RT-qPCR Kits Market Exclusive Report with Detailed Study Analysis

The One-Step RT-qPCR Kits market is growing steadily due to rising demand for rapid and accurate RNA detection in infectious disease diagnostics, gene expression studies, and clinical research. Increased adoption in hospitals, labs, and biotech companies, along with advances in RNA-based testing, continues to drive market expansion globally.
Published 17 November 2025

InsightAce Analytic Pvt. Ltd. announces the release of a market assessment report on the " One Step RT-qPCR Kits Market – (By Reaction (25 Rxn one step RT-qPCR kits, 50 Rxn one step RT-qPCR kits, 100 Rxn one step RT-qPCR kits, 200 Rxn one step RT-qPCR kits, 400 Rxn one step RT-qPCR kits, 500 Rxn one step RT-qPCR kits, 1000 Rxn one step RT-qPCR kits, and above 1000 Rxn one-step RT-qPCR kits), By Application (Virus Detection, Gene Expression Analysis, High-Sensitivity Detection & Quantification of RNA, Genetic Profiling, Mutation Detection, Multiplexing, Single Cell RT-PCR), By Detection (Probe-based Detection, Dye-based Detection), By End-User (Hospitals, Diagnostic Laboratories, Reference Laboratories, Academic and Research Institutes, and Other Facilities)), Trends, Industry Competition Analysis, Revenue and Forecast To 2031."

According to the latest research by InsightAce Analytic, the One Step RT-qPCR Kits Market is valued at US$ 109.3 Mn in 2023, and it is expected to reach US$ 152.0 Mn by 2031, with a CAGR of 4.3% during the forecast period of 2024-2031.

 

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One-step RT-qPCR kits are widely utilized for the simultaneous processing of multiple samples, each targeting one or more specific genetic sequences. These kits play a crucial role in various applications, including RNA quantification, viral detection and measurement, high-throughput gene expression analysis, and other molecular biology procedures.

The market for one-step RT-qPCR kits is experiencing significant growth, driven by the increasing demand for rapid diagnostic solutions, the expansion of research initiatives focused on developing innovative DNA and RNA-based therapeutics, and advancements in molecular testing technologies. However, challenges such as concerns over accuracy and repeatability are expected to hinder the global market's growth trajectory.

List of Prominent Players in the One-Step RT-qPCR Kits Market:

·      Takara Bio Inc.

·      Bio-Rad Laboratories, Inc.

·      Promega Corporation

·      Thermo Fisher Scientific Inc.

·      QIAGEN

·      TIANGEN Biotech(Beijing)Co., Ltd

·      EnzyQuest

·      Canvax

·      New England Biolabs

·      MP Biomedicals

·      Avantor, Inc.

·      Intact Genomics, Inc.

·      Fortis Life Sciences

·      Quantabio

·      BIO-HELIX

·      Abbexa

·      Boca Scientific Inc.

Market Dynamics:

Drivers- 

The demand for one-step RT-qPCR kits is primarily driven by the increasing need for accurate diagnostic solutions, particularly in response to the emergence of infectious diseases such as COVID-19. These kits play a critical role in detecting and quantifying nucleic acids, making them essential for diagnosing infections and monitoring disease progression. Additionally, the growing focus on point-of-care diagnostics, which requires highly sensitive yet user-friendly testing solutions, is encouraging continuous innovation from manufacturers.

 

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Challenges:

While one-step RT-qPCR kits remain a critical tool in molecular diagnostics, emerging alternative technologies such as CRISPR-based assays are posing a challenge to market growth. Originally developed for gene editing, CRISPR technology has been adapted for diagnostic applications through platforms such as DETECTR (DNA Endonuclease-Targeted CRISPR Trans Reporter) and SHERLOCK (Specific High-Sensitivity Enzymatic Reporter Unlocking). These advanced assays enable rapid and highly precise nucleic acid detection.

Regional Trends:

North America is expected to hold a substantial share of the one-step RT-qPCR kit market, driven by a strong focus on innovation in molecular diagnostics. Government initiatives such as the FDA’s Breakthrough Devices Program are accelerating the approval of cutting-edge diagnostic products. The introduction of FDA-approved rapid antigen tests during the COVID-19 pandemic further accelerated the adoption of at-home testing solutions, enabling individuals to self-diagnose and promoting a shift towards proactive healthcare.

 

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Segmentation of One Step RT-qPCR Kits Market-

By Reaction-

  • 25 Rxn one step RT-qPCR kits
  • 50 Rxn one step RT-qPCR kits
  • 100 Rxn one step RT-qPCR kits
  • 200 Rxn one step RT-qPCR kits
  • 400 Rxn one step RT-qPCR kits
  • 500 Rxn one step RT-qPCR kits
  • 1000 Rxn one step RT-qPCR kits
  • Above 1000 Rxn one step RT-qPCR kits

By Application-

  • Virus Detection
  • Gene Expression Analysis
  • High-Sensitivity Detection & Quantification of RNA
  • Genetic Profiling
  • Mutation Detection
  • Multiplexing
  • Single Cell RT-PCR

By Detection-

  • Probe-based Detection
  • Dye Based Detection

By End-User-

·      Hospitals

·      Diagnostic Laboratories

·      Reference Laboratories

·      Academic and Research Institutes

·      Others Facilities

By Region-

North America-

·      The US

·      Canada

·      Mexico

Europe-

·      Germany

·      The UK

·      France

·      Italy

·      Spain

·      Rest of Europe

Asia-Pacific-

·      China

·      Japan

·      India

·      South Korea

·      South East Asia

·      Rest of Asia Pacific

Latin America-

·      Brazil

·      Argentina

·      Rest of Latin America

 Middle East & Africa-

·      GCC Countries

·      South Africa

·      Rest of the Middle East and Africa

 

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InsightAce Analytic is a market research and consulting firm that enables clients to make strategic decisions. Our qualitative and quantitative market intelligence solutions inform the need for market and competitive intelligence to expand businesses. We help clients gain a competitive advantage by identifying untapped markets, exploring new and competing technologies, segmenting potential markets, and repositioning products. Our expertise is in providing syndicated and custom market intelligence reports with an in-depth analysis with key market insights in a timely and cost-effective manner.https://www.insightaceanalytic.com/images_data/148861653.

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